14-3-3 eta/YWHAH Monoclonal Antibody

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For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD269213A
Immunoprecipitation analysis using 2 µL anti-YWHAH mouse Monoclonal Antibody and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using YWHAH mouse Monoclonal Antibody at a dilution of 1:500. Lane A:0.5 mg Jurkat Whole Cell Lysate, Lane B:0.5 mg k562 Whole Cell Lysate
Immunoprecipitation analysis using 2 µL anti-YWHAH mouse Monoclonal Antibody and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using YWHAH mouse Monoclonal Antibody at a dilution of 1:500. Lane A:0.5 mg Jurkat Whole Cell Lysate, Lane B:0.5 mg k562 Whole Cell Lysate
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Overview

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Product Name
14-3-3 eta/YWHAH Monoclonal Antibody
Catalog Number
RD269213A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG1
Host
Mouse

Background

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This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 2' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder.

Immunogen Information

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Immunogen
Recombinant Human 14-3-3 eta / YWHAH protein
Swissprot
Q04917
Synonyms
YWHAYWHAHYWHA1
Calculated MW
28 kDa
Observed MW
30 kDa

Applications

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Reactivity
Human
Tested Applications
WB,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000;IP 1-4 μL/mg of lysate
Concentration
1 mg/mL
Storage Buffer
0.2 μm filtered solution in PBS
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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