AF647-conjugated beta actin Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
1X10⁶ C6 cells were intracellularly stained with 0.2 μg AF647-conjugated beta actin Monoclonal Antibody (red), or 0.2 μg AF647-conjugated beta actin Monoclonal Antibody Isotype Control (green).
1X10⁶ C6 cells were intracellularly stained with 0.2 μg AF647-conjugated beta actin Monoclonal Antibody (red), or 0.2 μg AF647-conjugated beta actin Monoclonal Antibody Isotype Control (green).
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Overview

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Product Name
AF647-conjugated beta actin Monoclonal Antibody
Catalog Number
RD269008A
Clonality
Monoclonal
Purification Method
Protein A/G Purification
Isotype
IgG1
Host

Background

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Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells, and commonly used as a loading control for western blot and as a control for qPCR.

Immunogen Information

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Immunogen
Recombinant Human Beta actin protein by E. Coli
Swissprot
P60709
Synonyms
ACTBActin betaActxActin cytoplasmic 1b actinA26C1A

Applications

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Reactivity
Rat
Tested Applications
FC
Conjugation
AF647
Dilution
FC 0.2 μg per 10⁶ cells in a 100 μL suspension
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide;1% protective protein and 50% glycerol;pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

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