alpha Tubulin Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western Blotting with anti-TUBA monoclonal antibody at dilution of 1:3000.
Lane 1: Scallop Muscle lysates, Lane 2: Zebrafish lysates
Western Blotting with anti-TUBA monoclonal antibody at dilution of 1:3000. Lane 1: Scallop Muscle lysates, Lane 2: Zebrafish lysates
Western Blotting with anti-TUBA monoclonal antibody at dilution of 1:2000.
Lane 1: Hela cell lysates, Lane 2:jurkat cell lysates, Lane, 3: 293T lysates, Lane 4: MCF-7 cell lysates, Lane 5: HT-29 cell lysates, Lane 6:HepG2 cell lysates, Lane 7:Rat kidney lysates
Western Blotting with anti-TUBA monoclonal antibody at dilution of 1:2000. Lane 1: Hela cell lysates, Lane 2:jurkat cell lysates, Lane, 3: 293T lysates, Lane 4: MCF-7 cell lysates, Lane 5: HT-29 cell lysates, Lane 6:HepG2 cell lysates, Lane 7:Rat kidney lysates
Western Blotting with anti-TUBA monoclonal antibody at dilution of 1:2000.
Lane 1: NIH/3T3 cell lysates, Lane 2:Raw264.7 cell lysates, Lane 3: Mouse heart lysates, Lane 4: Mouse spleen lysates, Lane 5: Mouse brain lysates, Lane 6:Mouse kidney lysates
Western Blotting with anti-TUBA monoclonal antibody at dilution of 1:2000. Lane 1: NIH/3T3 cell lysates, Lane 2:Raw264.7 cell lysates, Lane 3: Mouse heart lysates, Lane 4: Mouse spleen lysates, Lane 5: Mouse brain lysates, Lane 6:Mouse kidney lysates
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Overview

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Product Name
alpha Tubulin Monoclonal Antibody
Catalog Number
RD86714A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG2a
Host

Background

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Tubulin is a highly conserved αβ dimeric protein present and is essential for all eukaryotes. αβ-Tubulin dimers assemble into microtubules, dynamic polymers important in a variety of functions, most noticeably, mitosis. Tubulin assembly and disassembly are linked to guanosine triphosphate hydrolysis.

Immunogen Information

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Immunogen
Recombinant human TUBA protein
Gene ID
7846/10376
Swissprot
Q71U36/P68363
Synonyms
Alpha tubulin 3Alpha-tubulin 3B alpha 1FLJ25113LIS3TBA1ATUBA1ATUBA3Tubulin alpha 1aTubulin alpha 1A chainTubulin alpha 3Tubulin alpha 3 chainTubulin alpha brain specificTubulin alpha-1A chainTubulin alpha-3 chainTubulin B alp
Calculated MW
30kDa
Observed MW
36kDa

Applications

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Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:2000-1:5000
Concentration
5mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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