Firefly Luciferase Rabbit mAb

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot analysis of Firefly Luciferase Antibody expression in (1) Firefly Luciferase transfected 293T cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate.
Western blot analysis of Firefly Luciferase Antibody expression in (1) Firefly Luciferase transfected 293T cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate.
Immunofluorescent analysis of 293 cells transfected with Firefly Luciferase, using Firefly Luciferase Antibody.
Immunofluorescent analysis of 293 cells transfected with Firefly Luciferase, using Firefly Luciferase Antibody.
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Overview

Circle
Product Name
Firefly Luciferase Rabbit mAb
Catalog Number
RDSA66704
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG

Background

Circle
Produces green light with a wavelength of 562 nm. Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Immunogen Information

Circle
Immunogen
A synthesized peptide derived from Firefly Luciferase
Swissprot
P08659
Synonyms
Luciferase;Firefly;Luciferin 4 monooxygenase

Applications

Circle
Reactivity
Tested Applications
Conjugation
Unconjugated
Dilution
WB 1:1000-1:5000, ICC/IF 1:50-1:200
Storage Buffer
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

Research Areas

Circle

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