FITC-conjugated Vinculin Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
1X10⁶ Hela cells were intracellularly stained with 0.2 μg FITC-conjugated Vinculin Monoclonal Antibody(red), or 0.2 μg FITC-conjugated Vinculin Monoclonal Antibody Isotype Control (green).
1X10⁶ Hela cells were intracellularly stained with 0.2 μg FITC-conjugated Vinculin Monoclonal Antibody(red), or 0.2 μg FITC-conjugated Vinculin Monoclonal Antibody Isotype Control (green).
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Overview

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Product Name
FITC-conjugated Vinculin Monoclonal Antibody
Catalog Number
RD269012A
Clonality
Monoclonal
Purification Method
Protein A/G Purification
Isotype
IgG1
Host

Background

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Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1, 066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Immunogen Information

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Immunogen
Recombinant Human Vinculin protein expressed by E.coli
Swissprot
P18206
Synonyms
VinculinMetavinculin (MV)VCL

Applications

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Reactivity
Tested Applications
FC
Conjugation
FITC
Dilution
FC 0.2 μg per 10⁶ cells in a 100 μL suspension
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide and 0.1% gelatin;pH 7.4
Storage Instructions
Store at 4°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

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