HRP-conjugated beta Tubulin Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot with HRP-conjugated beta Tubulin Monoclonal Antibody at dilution of 1:5000.lane 1:Raji whole cell lysate,lane 2:.Jurkat whole cell lysate,lane 3: HL-60 whole cell lysate ,lane 4:U87-MG whole cell lysate,lane 5:mouse lung whole cell lysate,lane 6:Raw264.7 whole cell lysate, lane 7:C6 whole cell lysate,lane 8:zebrafish whole cell lysate ,lane 9:zebrafish whole cell lysate
Western blot with HRP-conjugated beta Tubulin Monoclonal Antibody at dilution of 1:5000.lane 1:Raji whole cell lysate,lane 2:.Jurkat whole cell lysate,lane 3: HL-60 whole cell lysate ,lane 4:U87-MG whole cell lysate,lane 5:mouse lung whole cell lysate,lane 6:Raw264.7 whole cell lysate, lane 7:C6 whole cell lysate,lane 8:zebrafish whole cell lysate ,lane 9:zebrafish whole cell lysate
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Overview

Circle
Product Name
HRP-conjugated beta Tubulin Monoclonal Antibody
Catalog Number
RD269003A
Clonality
Monoclonal
Purification Method
Protein A/G Purification
Isotype
IgG
Host

Background

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In eukaryotes there are six members of the tubulin superfamily. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes, including mitosis. Both α and β tubulins have a mass of around 50 kDa. Tubulin was long thought to be specific to eukaryotes. Beta-tubulin gene is commonly used as a loading control for western blot.

Immunogen Information

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Immunogen
Recombinant Human TUBB protein by E. Coli
Swissprot
P07437
Synonyms
Beta 4 tubulinBeta 5 tubulinbeta Ib tubulinBeta1 tubulinClass I beta tubulinTUBB 1TUBB 2TUBB 5TUBBTUBB1TUBB2TUBB5tubulin beta 1 chainTubulin beta 2 chaintubulin beta 5 chainTubulin beta chainTubulin beta class Itubulin beta polypeptideTu
Calculated MW
55kDa
Observed MW
55kDa

Applications

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Tested Applications
WB
Conjugation
HRP
Dilution
WB 1:2500-1:5000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.1% procline 300;1% protective protein and 50% glycerol;pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

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