MCP-3/CCL7 Monoclonal Antibody

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For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD269357A
Immunofluorescence analysis of Human CCL7 in SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human CCL7 monoclonal antibody (1:60). Then cells were stained with the AF488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.
Immunofluorescence analysis of Human CCL7 in SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human CCL7 monoclonal antibody (1:60). Then cells were stained with the AF488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.
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Overview

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Product Name
MCP-3/CCL7 Monoclonal Antibody
Catalog Number
RD269357A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG1
Host
Mouse

Background

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This gene encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q.

Immunogen Information

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Immunogen
Recombinant Human CCL7/MCP3 protein
Swissprot
P80098
Synonyms
CCLRP23-350GC-C motif chemokineChemokine CC motif ligandSmall-inducible cytokine AMonocyte chemotactic proteinMonocyte chemoattractant proteinMCPCCL7FICMARCMCP-3MCP3NC28SCYA6SCYA7C-C motif chemokine 7Chemokine CC motif ligand 7Monocyte c

Applications

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Reactivity
Human
Tested Applications
FC,ICC/IF
Conjugation
Unconjugated
Dilution
ICC/IF 1:20-1:100;FC 1:25-1:100
Concentration
1 mg/mL
Storage Buffer
0.2 μm filtered solution in PBS
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Research Areas

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Chapter Guide

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