Purified Anti-Mouse TNF-α Antibody[TN3-19.12]

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
HEK293T cells transfected with pcDNA3.1 plasmid encoding Mouse TNF-α gene were stained with 0.2 μg Purified Anti-Mouse TNF-α Antibody[TN3-19.12] (Right) and 0.2 μg Armenian Hamster IgG2, κ Isotype Control (Left), followed by APC-conjugated Goat Anti-Armenian Hamster IgG Secondary Antibody.
HEK293T cells transfected with pcDNA3.1 plasmid encoding Mouse TNF-α gene were stained with 0.2 μg Purified Anti-Mouse TNF-α Antibody[TN3-19.12] (Right) and 0.2 μg Armenian Hamster IgG2, κ Isotype Control (Left), followed by APC-conjugated Goat Anti-Armenian Hamster IgG Secondary Antibody.
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Overview

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Product Name
Purified Anti-Mouse TNF-α Antibody[TN3-19.12]
Catalog Number
RD32383F
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
Armenian Hamster IgG2, κ

Background

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Tumor necrosis factor alpha (TNF-alpha, TNF- alpha, TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Mouse TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD).

Immunogen Information

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Swissprot
P06804
Synonyms
Tumor necrosis factor ligand superfamily memberTNFSFDIFTnfaTNF-aTNFSF2Tnlg1fTnfsf1aTNFalphaTNF-alphaICD1ICD2Membrane FormN-terminal fragmentNTFTnfCachectinTumor necrosis factorTumor necrosis factor ligand superfamily member 2TnfaTNF-a

Applications

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Reactivity
Tested Applications
FC
Conjugation
Unconjugated
Dilution
FC 2 μg/mL(0.5×10⁶-1×10⁶ cells)
Concentration
≥ 1 mg/mL
Storage Buffer
Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.
Storage Instructions
Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.

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