Recombinant Alkaline Phosphatase/ALPL Monoclonal Antibody

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For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD269485A
Western Blot with Alkaline Phosphatase / ALPL Monoclonal Antibody at dilution of 1:500. Lane A: Hela Whole Cell Lysate, Lysates/proteins at 30 μg per lane.
Western Blot with Alkaline Phosphatase / ALPL Monoclonal Antibody at dilution of 1:500. Lane A: Hela Whole Cell Lysate, Lysates/proteins at 30 μg per lane.
Western Blot with Alkaline Phosphatase / ALPL Monoclonal Antibody at dilution of 1:500. Lane A: ALPL konckout Hela Whole Cell Lysate, Lane B: Hela Whole Cell Lysate, Lysates/proteins at 20 μg per lane.
Western Blot with Alkaline Phosphatase / ALPL Monoclonal Antibody at dilution of 1:500. Lane A: ALPL konckout Hela Whole Cell Lysate, Lane B: Hela Whole Cell Lysate, Lysates/proteins at 20 μg per lane.
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Overview

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Product Name
Recombinant Alkaline Phosphatase/ALPL Monoclonal Antibody
Catalog Number
RD269485A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Rabbit

Background

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The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

Immunogen Information

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Immunogen
A synthetic peptide corresponding to the center region of the Human Alkaline Phosphatase / ALPL
Swissprot
P05186
Synonyms
ALPLAP-TNAPAPTNAPHOPSTNAPTNSALPPPBTAlkaline phosphatasetissue-nonspecific isozymeAlkaline phosphatase liver/bone/kidney isozymeAKP2Alkaline phosphatase liver/bone/kidneyAlkaline phosphatase tissue nonspecific isozymeAlkaline phosphomonoestera
Calculated MW
57 kDa
Observed MW
95 kDa

Applications

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Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000;
Concentration
1 mg/mL
Storage Buffer
0.2 μm filtered solution in PBS
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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