Recombinant Bid Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western Blot with Bid Monoclonal Antibody at dilution of 1:2000. Lane 1: Jurkat, Lane 2: Raji, Lane 3: RAW264.7, Lane 4: PC-12
Western Blot with Bid Monoclonal Antibody at dilution of 1:2000. Lane 1: Jurkat, Lane 2: Raji, Lane 3: RAW264.7, Lane 4: PC-12
Immunohistochemistry of paraffin-embedded Human colon cancer using Bid Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human colon cancer using Bid Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Mouse small intestine using Bid Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Mouse small intestine using Bid Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat colon using Bid Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat colon using Bid Monoclonal Antibody at dilution of 1:1000.
Immunoprecipitation analysis using anti-Bid Monoclonal Antibody. Western blot was performed from the immunoprecipitate using Bid Monoclonal Antibody at a dilution of 1:100. Lane 1: 5% Input, Lane 2: Bid Monoclonal Antibody, Lane 3: Rabbit monoclonal IgG Isotype
Immunoprecipitation analysis using anti-Bid Monoclonal Antibody. Western blot was performed from the immunoprecipitate using Bid Monoclonal Antibody at a dilution of 1:100. Lane 1: 5% Input, Lane 2: Bid Monoclonal Antibody, Lane 3: Rabbit monoclonal IgG Isotype
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Overview

Circle
Product Name
Recombinant Bid Monoclonal Antibody
Catalog Number
RD270842A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG; κ

Background

Circle
The major proteolytic product p15 BID allows the release of cytochrome c . Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

Immunogen Information

Circle
Immunogen
Recombinant human Bid fragment
Swissprot
P55957
Synonyms
BIDFP497BH3-Interacting Domain Death Agonistp22 BID
Calculated MW
22 kDa
Observed MW
17-26 kDa

Applications

Circle
Reactivity
Tested Applications
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000;IHC 1:200-1:1000;IP 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS; 50% glycerol; 0.05% Proclin 300; 0.05% protein protectant.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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