Recombinant ENO3/beta-enolase Monoclonal Antibody

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For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD269492A
Immunohistochemistry of paraffin-embedded human skeletal muscle using ENO3 / beta-enolase Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded human skeletal muscle using ENO3 / beta-enolase Monoclonal Antibody at dilution of 1:200.
Immunoprecipitation analysis using 2 µL anti-ENO3 Monoclonal Antibody and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using ENO3 Monoclonal Antibody at a dilution of 1:100. Lane A:0.5 mg Raji Whole Cell Lysate
Immunoprecipitation analysis using 2 µL anti-ENO3 Monoclonal Antibody and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using ENO3 Monoclonal Antibody at a dilution of 1:100. Lane A:0.5 mg Raji Whole Cell Lysate
Western Blot with ENO3 / beta-enolase Monoclonal Antibody at dilution of 1:500. Lane A: HepG2 Whole Cell Lysate, Lane B: Raji Whole Cell Lysate, Lane C: Hela Whole Cell Lysate, Lane D: MOLT-4 Whole Cell Lysate, Lysates/proteins at 30 μg per lane.
Western Blot with ENO3 / beta-enolase Monoclonal Antibody at dilution of 1:500. Lane A: HepG2 Whole Cell Lysate, Lane B: Raji Whole Cell Lysate, Lane C: Hela Whole Cell Lysate, Lane D: MOLT-4 Whole Cell Lysate, Lysates/proteins at 30 μg per lane.
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Overview

Circle
Product Name
Recombinant ENO3/beta-enolase Monoclonal Antibody
Catalog Number
RD269492A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.

Immunogen Information

Circle
Immunogen
Recombinant Human ENO3 / beta-enolase Protein
Swissprot
P13929
Synonyms
GSDENOenolaseENO3GSD13MSEenolase 32 phospho D glycerate hydrol yase2 phospho D glycerate hydrolyase2-phospho-D-glycerate hydro-lyaseBeta enolaseBeta-enolaseENOBEnolase 3 (beta muscle)Enolase3Muscle specific enolaseMuscle-specific enolaseS
Calculated MW
47 kDa
Observed MW
53 kDa

Applications

Circle
Reactivity
Human
Tested Applications
WB,IHC-P,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000;IHC-P 1:100-1:500;IP 1-4 μL/mg of lysate
Concentration
1 mg/mL
Storage Buffer
0.2 μm filtered solution in PBS
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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