Recombinant FDPS/Farnesyl Diphosphate Synthase Monoclonal Antibody

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Catalog Number
RD269472A
Flow cytometric analysis of Human FDPS expression on HeLa cells. The cells were stained with purified anti-Human FDPS, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Flow cytometric analysis of Human FDPS expression on HeLa cells. The cells were stained with purified anti-Human FDPS, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human FDPS in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human FDPS Monoclonal Antibody (1:60) at 37℃ 1 hour. Then cells were stained with the AF488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.
Immunofluorescence analysis of Human FDPS in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human FDPS Monoclonal Antibody (1:60) at 37℃ 1 hour. Then cells were stained with the AF488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.
Immunoprecipitation analysis using 2 µL anti-FDPS Monoclonal Antibody and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using FDPS Monoclonal Antibody at a dilution of 1:100. Lane A:0.5 mg Hela Whole Cell Lysate, Lane B:0.5 mg HepG2 Whole Cell Lysate, Lane C:0.5 mg A549 Whole Cell Lysate
Immunoprecipitation analysis using 2 µL anti-FDPS Monoclonal Antibody and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using FDPS Monoclonal Antibody at a dilution of 1:100. Lane A:0.5 mg Hela Whole Cell Lysate, Lane B:0.5 mg HepG2 Whole Cell Lysate, Lane C:0.5 mg A549 Whole Cell Lysate
Western Blot with FDPS / Farnesyl Diphosphate Synthase Monoclonal Antibody at dilution of 1:500. Lane A: HepG2 Whole Cell Lysate, Lane B: Hela Whole Cell Lysate, Lysates/proteins at 30 μg per lane.
Western Blot with FDPS / Farnesyl Diphosphate Synthase Monoclonal Antibody at dilution of 1:500. Lane A: HepG2 Whole Cell Lysate, Lane B: Hela Whole Cell Lysate, Lysates/proteins at 30 μg per lane.
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Overview

Circle
Product Name
Recombinant FDPS/Farnesyl Diphosphate Synthase Monoclonal Antibody
Catalog Number
RD269472A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene encodes an enzyme that catalyzes the production of geranyl pyrophosphate and farnesyl pyrophosphate from isopentenyl pyrophosphate and dimethylallyl pyrophosphate. The resulting product, farnesyl pyrophosphate, is a key intermediate in cholesterol and sterol biosynthesis, a substrate for protein farnesylation and geranylgeranylation, and a ligand or agonist for certain hormone receptors and growth receptors. Drugs that inhibit this enzyme prevent the post-translational modifications of small GTPases and have been used to treat diseases related to bone resorption. Multiple pseudogenes have been found on chromosomes 1, 7, 14, 15, 18 and X. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Circle
Immunogen
Recombinant Human FDPS / Farnesyl Diphosphate Synthase protein
Swissprot
P14324
Synonyms
KIAAPOROKFDPSFPPSFPSPOROK9DimethylallyltranstransferaseFarnesyl diphosphate synthaseFarnesyl diphosphate synthetaseFarnesyl pyrophosphate synthaseFarnesyl pyrophosphate synthetaseFPP synthaseFPP synthetaseFPPS_HUMANGeranyltranstransferaseKI
Calculated MW
48 kDa
Observed MW
38 kDa

Applications

Circle
Reactivity
Human
Tested Applications
WB,FC,ICC/IF,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000;FC 1:25-1:100;ICC/IF 1:20-1:100;IP 1-4 μL/mg of lysate
Concentration
1 mg/mL
Storage Buffer
0.2 μm filtered solution in PBS
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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