Recombinant JAK2 Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-JAK2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Predicted band size: 131kDa Observed band size: 131kDa
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-JAK2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Predicted band size: 131kDa Observed band size: 131kDa
Human lung carcinoma was stained with anti-JAK2 rabbit antibody
Human lung carcinoma was stained with anti-JAK2 rabbit antibody
Immunofluorescence analysis of HEK293. Picture A: JAK2 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B
Immunofluorescence analysis of HEK293. Picture A: JAK2 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B
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Overview

Circle
Product Name
Recombinant JAK2 Monoclonal Antibody
Catalog Number
RD270250A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG;κ

Background

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This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis.

Immunogen Information

Circle
Immunogen
Recombinant Human JAK2 protein
Swissprot
O60674
Synonyms
kinase JakJanus kinaseJanus Activating KinaseTyrosine protein kinase JAKTyrosine-protein kinase JAKTHCYTOTTHUMPJTKJAKJAK2JTK10THCYT3JAK 2JAK-2Janus Activating Kinase 2Janus kinase 2Janus kinase 2 (a protein tyrosine kinase)JTK 10kinase J
Calculated MW
131 kDa
Observed MW
131 kDa

Applications

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Reactivity
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:2000-1:10000
Concentration
0.2 mg/mL
Storage Buffer
PBS; 50% glycerol; 0.05% Proclin 300; 0.05% protein protectant.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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