Recombinant Phospho-4E-BP1 (Thr37, 46) Monoclonal Antibody

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Catalog Number
RD269445A
Western blot analysis of extracts from serum-starved HEK293, untreated (line A); treated with IGF1 (100ng/ml, 15min；+) (line B); treated with IGF1 and λ-phosphatase (line C) using Phospho-4E-BP1 (Thr37, 46) Monoclonal Antibody at 1:5000 dilution.
Western blot analysis of extracts from serum-starved HEK293, untreated (line A); treated with IGF1 (100ng/ml, 15min；+) (line B); treated with IGF1 and λ-phosphatase (line C) using Phospho-4E-BP1 (Thr37, 46) Monoclonal Antibody at 1:5000 dilution.
Western blot analysis of extracts from HEK293, untreated(line A) or treated with IGF1 (100ng/ml, 15min; +)(line B), using Phospho-4E-BP1 (Thr37, 46) Monoclonal Antibody at 1:5000 dilution (upper) or 4E-BP1 / EIF4EBP1 Monoclonal Antibody at 1:5000 dilution (lower).
Western blot analysis of extracts from HEK293, untreated(line A) or treated with IGF1 (100ng/ml, 15min; +)(line B), using Phospho-4E-BP1 (Thr37, 46) Monoclonal Antibody at 1:5000 dilution (upper) or 4E-BP1 / EIF4EBP1 Monoclonal Antibody at 1:5000 dilution (lower).
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Overview

Circle
Product Name
Recombinant Phospho-4E-BP1 (Thr37, 46) Monoclonal Antibody
Catalog Number
RD269445A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Rabbit

Background

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This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Immunogen Information

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Immunogen
A synthetic peptide corresponding to residues around (Thr37; 46) of Human Phospho-4E-BP1
Swissprot
Q13541
Synonyms
4EBPeIF4E binding proteineukaryotic translation initiation factor 4E-binding protein4E-BPEIF4EBPEIF4EBP14E-BP14EBP1BP-1PHAS-Ieukaryotic translation initiation factor 4E-binding protein 1BP 1eIF4E binding protein 1eIF4E-binding protein 1PHASI
Calculated MW
13 kDa
Observed MW
17 kDa

Applications

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Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:1000-1:10000;
Concentration
1 mg/mL
Storage Buffer
10 mM sodium HEPES; 150 mM NaCl; 100 μg/mL protein protectant; 50% glycerol; pH 7.5
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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