Recombinant Phospho-CREB (Ser133) Monoclonal Antibody

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For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD269519A
Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-CREB (Ser133) Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-CREB (Ser133) Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-CREB (Ser133) Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-CREB (Ser133) Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded human renal carcinoma using Phospho-CREB (Ser133) Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded human renal carcinoma using Phospho-CREB (Ser133) Monoclonal Antibody at dilution of 1:200.
Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with TPA (200 nM, 15 min; +), using Phospho-CREB (Ser133) Monoclonal Antibody at 1:200000 dilution (upper) or Anti-CREB Monoclonal Antibody at 1:500 dilution (middle) or Beta-Tubulin Loading Control Antibody (Chimeric) at 1:40000 dilution (lower). Phospho-CREB (Ser133) Monoclonal Antibody may cross with Phospho-ATF-1 (Ser63) by sequence similarity.
Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with TPA (200 nM, 15 min; +), using Phospho-CREB (Ser133) Monoclonal Antibody at 1:200000 dilution (upper) or Anti-CREB Monoclonal Antibody at 1:500 dilution (middle) or Beta-Tubulin Loading Control Antibody (Chimeric) at 1:40000 dilution (lower). Phospho-CREB (Ser133) Monoclonal Antibody may cross with Phospho-ATF-1 (Ser63) by sequence similarity.
Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with TPA (100 nM, 15 min) (line B); treated with TPA and λ-phosphatase (line C) using Phospho-CREB (Ser133) Monoclonal Antibody at 1:200000 dilution.
Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with TPA (100 nM, 15 min) (line B); treated with TPA and λ-phosphatase (line C) using Phospho-CREB (Ser133) Monoclonal Antibody at 1:200000 dilution.
Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with TPA (100 nM, 15 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-CREB (Ser133) Monoclonal Antibody at 1:200000 dilution.
Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with TPA (100 nM, 15 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-CREB (Ser133) Monoclonal Antibody at 1:200000 dilution.
Western blot analysis of extracts from serum-starved Hela treated with TPA (100 nM, 15 min), using Phospho-CREB (Ser133) Monoclonal Antibody at 1:10000, 1:100000, 1:500000 dilution.
Western blot analysis of extracts from serum-starved Hela treated with TPA (100 nM, 15 min), using Phospho-CREB (Ser133) Monoclonal Antibody at 1:10000, 1:100000, 1:500000 dilution.
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Overview

Circle
Product Name
Recombinant Phospho-CREB (Ser133) Monoclonal Antibody
Catalog Number
RD269519A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.

Immunogen Information

Circle
Immunogen
A synthetic phosphopeptide corresponding to residues around Ser133 of human CREB.
Swissprot
P16220
Synonyms
CREB1CREBCREB-1Active transcription factor CREBcAMP response element binding proteincAMP response element binding protein 1cAMP responsive element binding protein 1cAMP-responsive element-binding protein 1Cyclic AMP-responsive element-binding prot
Calculated MW
35 kDa
Observed MW
38 kDa, 40 kDa

Applications

Circle
Reactivity
Human
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:10000-1:200000;IHC-P 1:100-1:500;
Concentration
1 mg/mL
Storage Buffer
10 mM sodium HEPES; 150 mM NaCl; 100 μg/mL protein protectant; 50% glycerol; pH 7.5
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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