Recombinant Phospho-MAPKAPK-2 (Thr334) Monoclonal Antibody

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Catalog Number
RD269549A
Western blot analysis of extracts from serum-starved A431, untreated(line A) or treated with calyculin A (100mM, 30min; +)(line B), using Phospho-MAPKAPK-2 (Thr334) Monoclonal Antibody at 1:200 dilution (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal at 1:50000 dilution (lower).
Western blot analysis of extracts from serum-starved A431, untreated(line A) or treated with calyculin A (100mM, 30min; +)(line B), using Phospho-MAPKAPK-2 (Thr334) Monoclonal Antibody at 1:200 dilution (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal at 1:50000 dilution (lower).
Western blot analysis of extracts from serum-starved A431, untreated (line A); treated with calyculin A (100mM, 30min; +) (line B); treated with calyculin A and λ-phosphatase (line C) using Phospho-MAPKAPK-2 (Thr334) Monoclonal Antibody at 1:200 dilution.
Western blot analysis of extracts from serum-starved A431, untreated (line A); treated with calyculin A (100mM, 30min; +) (line B); treated with calyculin A and λ-phosphatase (line C) using Phospho-MAPKAPK-2 (Thr334) Monoclonal Antibody at 1:200 dilution.
Western blot analysis of extracts from serum-starved A431, untreated (line A); treated with calyculin A (100mM, 30min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-MAPKAPK-2 (Thr334) Monoclonal Antibody at 1:200 dilution.
Western blot analysis of extracts from serum-starved A431, untreated (line A); treated with calyculin A (100mM, 30min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-MAPKAPK-2 (Thr334) Monoclonal Antibody at 1:200 dilution.
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Overview

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Product Name
Recombinant Phospho-MAPKAPK-2 (Thr334) Monoclonal Antibody
Catalog Number
RD269549A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Rabbit

Background

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This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP24 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Immunogen Information

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Immunogen
A synthetic peptide corresponding to the residues around (Thr334) of Human Phospho-MAPKAPK-2
Swissprot
P49137
Synonyms
MAPKAPKMAPKAP-KMAPKAPK2MAPKAP-K2MK-2MK2
Calculated MW
45 kDa
Observed MW
45 kDa;40 kDa

Applications

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Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000;
Concentration
1 mg/mL
Storage Buffer
10 mM sodium HEPES; 150 mM NaCl; 100 μg/mL protein protectant; 50% glycerol; pH 7.5
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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