Recombinant Phospho-Src (Tyr530) Monoclonal Antibody

Request Manual FAQ

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD269782A
Western blot analysis of extracts from serum-starved Jurkat, untreated(line A) or treated with H2O2 (2mM, 2min; +)(line B), using Phospho-Src (Tyr530) rabbit monoclonal Antibody at 1:2000 dilution. (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal at 1:50000 dilution(lower).
Western blot analysis of extracts from serum-starved Jurkat, untreated(line A) or treated with H2O2 (2mM, 2min; +)(line B), using Phospho-Src (Tyr530) rabbit monoclonal Antibody at 1:2000 dilution. (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal at 1:50000 dilution(lower).
Western blot analysis of extracts from serum-starved Jurkat, untreated (line A); treated with H2O2 (2mM, 2min; +) (line B); treated with H2O2 and λ-phosphatase (line C) using Phospho-Src (Tyr530) rabbit monoclonal Antibody at 1:2000 dilution.
Western blot analysis of extracts from serum-starved Jurkat, untreated (line A); treated with H2O2 (2mM, 2min; +) (line B); treated with H2O2 and λ-phosphatase (line C) using Phospho-Src (Tyr530) rabbit monoclonal Antibody at 1:2000 dilution.
Western blot analysis of extracts from serum-starved Jurkat treated with H2O2 (2mM, 2min), using Phospho-Src (Tyr530) rabbit monoclonal Antibody at 1:10000, 1:50000, 1:200000 dilution.
Western blot analysis of extracts from serum-starved Jurkat treated with H2O2 (2mM, 2min), using Phospho-Src (Tyr530) rabbit monoclonal Antibody at 1:10000, 1:50000, 1:200000 dilution.
Add to Cart

For more information on our kits, please

Overview

Circle
Product Name
Recombinant Phospho-Src (Tyr530) Monoclonal Antibody
Catalog Number
RD269782A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Immunogen Information

Circle
Immunogen
A synthetic peptide corresponding to the residues around
Swissprot
P12931
Synonyms
THCASVSRC1THC6c-SRCp60-SrcSRCpp60c-srcProto-oncogene c-SrcProto-oncogene tyrosine-protein kinase SrcAvian sarcoma virusc SRCCDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNAcSrcE
Calculated MW
60 kDa
Observed MW
60 kDa

Applications

Circle
Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:1000-1:10000
Concentration
1 mg/mL
Storage Buffer
10 mM sodium HEPES; 150 mM NaCl; 100 μg/mL protein protectant; 50% glycerol; pH 7.5
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Chapter Guide

Your item has been successfully added to your cart .

Item # Price
Proceed to Cart
Never miss an update.

Sign up for our monthly newsletter to hear about exclusive sales and product tips and tricks.