Recombinant Phospho-VEGF Receptor 2 (Tyr1175) Monoclonal Antibody

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Catalog Number
RD269781A
Western blot analysis of extracts from serum-starved HUVEC, untreated(line A) or treated with VEGF (50ng/mL, 2min; +)(line B), using Phospho-VEGF Receptor 2 (Tyr1175) rabbit monoclonal Antibody at 1:10000 dilution. (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal at 1:50000 dilution(lower).
Western blot analysis of extracts from serum-starved HUVEC, untreated(line A) or treated with VEGF (50ng/mL, 2min; +)(line B), using Phospho-VEGF Receptor 2 (Tyr1175) rabbit monoclonal Antibody at 1:10000 dilution. (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal at 1:50000 dilution(lower).
Western blot analysis of extracts from serum-starved HUVEC, untreated (line A); treated with VEGF (50ng/mL, 2min; +) (line B); treated with VEGF and λ-phosphatase (line C) using Phospho-VEGF Receptor 2 (Tyr1175) rabbit monoclonal Antibody at 1:10000 dilution.
Western blot analysis of extracts from serum-starved HUVEC, untreated (line A); treated with VEGF (50ng/mL, 2min; +) (line B); treated with VEGF and λ-phosphatase (line C) using Phospho-VEGF Receptor 2 (Tyr1175) rabbit monoclonal Antibody at 1:10000 dilution.
Western blot analysis of extracts from serum-starved HUVEC, untreated (line A); treated with VEGF (50ng/mL, 2min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-VEGF Receptor 2 (Tyr1175) rabbit monoclonal Antibody at 1:10000 dilution.
Western blot analysis of extracts from serum-starved HUVEC, untreated (line A); treated with VEGF (50ng/mL, 2min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-VEGF Receptor 2 (Tyr1175) rabbit monoclonal Antibody at 1:10000 dilution.
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Overview

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Product Name
Recombinant Phospho-VEGF Receptor 2 (Tyr1175) Monoclonal Antibody
Catalog Number
RD269781A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Rabbit

Background

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Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.

Immunogen Information

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Immunogen
A synthetic peptide corresponding to the residues around Tyr1175 of Human Phospho-VEGF Receptor 2
Swissprot
P35968
Synonyms
KrdVEGF ReceptorFetal liver kinaseProtein-tyrosine kinase receptor flkVascular endothelial growth factor receptorsVEGFRVEGF R6130401CFLKCD309FLK1VEGFRVEGFR2VEGF Receptor 2KDR6130401C07Fetal liver kinase 1FLK-1Kinase insert domain recepto
Calculated MW
152 kDa
Observed MW
280 kDa

Applications

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Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:1000-1:10000
Concentration
1 mg/mL
Storage Buffer
10 mM sodium HEPES; 150 mM NaCl; 100 μg/mL protein protectant; 50% glycerol; pH 7.5
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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