Recombinant PHPT1 Monoclonal Antibody

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For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD269651A
Flow cytometric analysis of Human PHPT1 expression on THP1 cells . The cells were stained with purified anti-Human PHPT1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Flow cytometric analysis of Human PHPT1 expression on THP1 cells . The cells were stained with purified anti-Human PHPT1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human PHPT1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PHPT1 Monoclonal Antibody (1:60) at 37℃ 1 hour. Then cells were stained with the AF488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.
Immunofluorescence analysis of Human PHPT1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PHPT1 Monoclonal Antibody (1:60) at 37℃ 1 hour. Then cells were stained with the AF488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.
Immunohistochemistry of paraffin-embedded human skeletal muscle using PHPT1 Monoclonal Antibody at dilution of 1:5000.
Immunohistochemistry of paraffin-embedded human skeletal muscle using PHPT1 Monoclonal Antibody at dilution of 1:5000.
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Overview

Circle
Product Name
Recombinant PHPT1 Monoclonal Antibody
Catalog Number
RD269651A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Rabbit

Background

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This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.2 channels. Alternative splicing results in multiple transcript variants.

Immunogen Information

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Immunogen
Recombinant Human PHPT1 protein
Swissprot
Q9NRX4
Synonyms
PHPTHSPCPhosphohistidine phosphataseCGIPHPT1CGI-202HEL-S-132PHSPC141PHPPHP1414 kDa phosphohistidine phosphataseCGI 202Phosphohistidine phosphatase 1Protein janus A homologProtein janus-A homologSex regulated protein janus a

Applications

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Reactivity
Human
Tested Applications
IHC-P,FC,ICC/IF
Conjugation
Unconjugated
Dilution
IHC-P 1:2500-1:10000;ICC/IF 1:20-1:100;FC 1:25-1:100;
Concentration
1 mg/mL
Storage Buffer
0.2 μm filtered solution in PBS
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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