Recombinant PHPT1 Monoclonal Antibody

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For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD269652A
Immunoprecipitation analysis using 4 µL anti-PHPT1 Monoclonal Antibody and 60 μg of Immunomagnetic beads Protein A/G. Western blot was performed from the immunoprecipitate using PHPT1 Monoclonal Antibody at a dilution of 1:100. Lane A:0.5 mg HepG2 Whole Cell Lysate
Immunoprecipitation analysis using 4 µL anti-PHPT1 Monoclonal Antibody and 60 μg of Immunomagnetic beads Protein A/G. Western blot was performed from the immunoprecipitate using PHPT1 Monoclonal Antibody at a dilution of 1:100. Lane A:0.5 mg HepG2 Whole Cell Lysate
Western Blot with PHPT1 Monoclonal Antibody at dilution of 1:500. Lane A: HepG2 Whole Cell Lysate, Lane B: THP1 Whole Cell Lysate, Lane C: MCF7 Whole Cell Lysate, Lysates/proteins at 30 μg per lane.
Western Blot with PHPT1 Monoclonal Antibody at dilution of 1:500. Lane A: HepG2 Whole Cell Lysate, Lane B: THP1 Whole Cell Lysate, Lane C: MCF7 Whole Cell Lysate, Lysates/proteins at 30 μg per lane.
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Overview

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Product Name
Recombinant PHPT1 Monoclonal Antibody
Catalog Number
RD269652A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Rabbit

Background

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This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.2 channels. Alternative splicing results in multiple transcript variants.

Immunogen Information

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Immunogen
Recombinant Human PHPT1 protein
Swissprot
Q9NRX4
Synonyms
PHPTHSPCPhosphohistidine phosphataseCGIPHPT1CGI-202HEL-S-132PHSPC141PHPPHP1414 kDa phosphohistidine phosphataseCGI 202Phosphohistidine phosphatase 1Protein janus A homologProtein janus-A homologSex regulated protein janus a
Calculated MW
14 kDa
Observed MW
14 kDa

Applications

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Reactivity
Human
Tested Applications
WB,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000;IP 1-5 µL/mg of lysate
Concentration
1 mg/mL
Storage Buffer
0.2 μm filtered solution in PBS
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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