Recombinant PMVK/phosphomevalonate kinase Monoclonal Antibody

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For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD269678A
Immunoprecipitation analysis using 0.5 µL anti-PMVK Monoclonal Antibody and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using PMVK Monoclonal Antibody at a dilution of 1:500. Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg NIH-3T3 Whole Cell Lysate
Immunoprecipitation analysis using 0.5 µL anti-PMVK Monoclonal Antibody and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using PMVK Monoclonal Antibody at a dilution of 1:500. Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg NIH-3T3 Whole Cell Lysate
Western Blot with PMVK / phosphomevalonate kinase Monoclonal Antibody at dilution of 1:500. Lane A: HepG2 Whole Cell Lysate, Lysates/proteins at 30 μg per lane.
Western Blot with PMVK / phosphomevalonate kinase Monoclonal Antibody at dilution of 1:500. Lane A: HepG2 Whole Cell Lysate, Lysates/proteins at 30 μg per lane.
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Overview

Circle
Product Name
Recombinant PMVK/phosphomevalonate kinase Monoclonal Antibody
Catalog Number
RD269678A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene encodes a peroxisomal enzyme that is a member of the galactokinase, homoserine kinase, mevalonate kinase, and phosphomevalonate kinase (GHMP) family of ATP-dependent enzymes. The encoded protein catalyzes the conversion of mevalonate 5-phosphate to mevalonate 2-diphosphate, which is the fifth step in the mevalonate pathway of isoprenoid biosynthesis. Mutations in this gene are linked to certain types of porokeratosis including disseminated superficial porokeratosis. Alternative splicing results in multiple transcript variants.

Immunogen Information

Circle
Immunogen
Recombinant Human PMVK / phosphomevalonate kinase Protein
Swissprot
Q15126
Synonyms
POROKOTTHUMPPMVKHUMPMKIPMKPMKAPMKASEPOROK1hPMKOTTHUMP00000035546Phosphomevalonate kinasePMK PENPMKPENPMKI
Calculated MW
21 kDa
Observed MW
21 kDa

Applications

Circle
Reactivity
Human
Tested Applications
WB,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000;IP 0.2-1 μL/mg of lysate
Concentration
1 mg/mL
Storage Buffer
0.2 μm filtered solution in PBS
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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