Recombinant PRAME Monoclonal Antibody

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For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD271196A
Western Blot with PRAME Monoclonal Antibody at dilution of 1:1000. Lane 1: Ramos, Lane 2: K562, Lane 3: HeLa, Lane 4: A375
Western Blot with PRAME Monoclonal Antibody at dilution of 1:1000. Lane 1: Ramos, Lane 2: K562, Lane 3: HeLa, Lane 4: A375
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Overview

Circle
Product Name
Recombinant PRAME Monoclonal Antibody
Catalog Number
RD271196A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG; κ
Host
Rabbit

Background

Circle
This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.

Immunogen Information

Circle
Immunogen
Recombinant human PRAME fragment
Swissprot
P78395
Synonyms
OIPPRAMECT130MAPEOIP-4OIP44930534P07RikCancer/testis antigen 130Melanoma antigen preferentially expressed in tumorsOIP 4OIP4OPA interacting protein 4Opa interacting protein OIP4OPA-interacting protein 4Preferentially expressed antigen in mel
Calculated MW
58 kDa
Observed MW
58 kDa

Applications

Circle
Reactivity
Human;
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
1 mg/mL
Storage Buffer
PBS; 50% glycerol; 0.05% Proclin 300; 0.05% protein protectant.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
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