Recombinant Purine nucleoside phosphorylase/PNP Monoclonal Antibody

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Catalog Number
RD269811A
Western Blot with PNP3 Monoclonal Antibody at dilution of 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: K562 Whole Cell Lysate, Lane C: 293T Whole Cell Lysate, Lysates/proteins at 30 μg per lane.
Western Blot with PNP3 Monoclonal Antibody at dilution of 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: K562 Whole Cell Lysate, Lane C: 293T Whole Cell Lysate, Lysates/proteins at 30 μg per lane.
Immunoprecipitation analysis using 2 µL anti-PNP-3 Monoclonal Antibody and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using PNP-3 Monoclonal Antibody at a dilution of 1:100. Lane A:0.5 mg Jurkat Whole Cell Lysate, Lane B:0.5 mg K562 Whole Cell Lysate
Immunoprecipitation analysis using 2 µL anti-PNP-3 Monoclonal Antibody and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using PNP-3 Monoclonal Antibody at a dilution of 1:100. Lane A:0.5 mg Jurkat Whole Cell Lysate, Lane B:0.5 mg K562 Whole Cell Lysate
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Overview

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Product Name
Recombinant Purine nucleoside phosphorylase/PNP Monoclonal Antibody
Catalog Number
RD269811A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Rabbit

Background

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This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.

Immunogen Information

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Immunogen
Recombinant Human Purine nucleoside phosphorylase/PNP protein
Swissprot
P00491
Synonyms
PROPNPNPPRO1837PUNP
Calculated MW
32 kDa
Observed MW
32 kDa

Applications

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Reactivity
Human
Tested Applications
WB,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000;IP 1-4 μL/mg of lysate
Concentration
1 mg/mL
Storage Buffer
0.2 μm filtered solution in PBS
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Research Areas

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Chapter Guide

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