Recombinant SMAC/Diablo Monoclonal Antibody

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Catalog Number
RD269471A
Flow cytometric analysis of Human Diablo expression on HeLa cells. The cells were stained with purified anti-Human Diablo (Filled hisgram), then a FITC-conjugated second step antibody. To demonstrate specificity of staining, the binding by 10339-R006 was blocked by preincubation of the purified antibody with 20ug recombinant human Diablo for 1 hour (Black solid line hisgram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Flow cytometric analysis of Human Diablo expression on HeLa cells. The cells were stained with purified anti-Human Diablo (Filled hisgram), then a FITC-conjugated second step antibody. To demonstrate specificity of staining, the binding by 10339-R006 was blocked by preincubation of the purified antibody with 20ug recombinant human Diablo for 1 hour (Black solid line hisgram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of Human Diablo in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Diablo Monoclonal Antibody (1:60) at 4℃ overnight. Then cells were stained with the AF488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.
Immunofluorescence analysis of Human Diablo in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Diablo Monoclonal Antibody (1:60) at 4℃ overnight. Then cells were stained with the AF488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI for nuclear staining (blue). Positive staining was localized to cytoplasm.
Western Blot with SMAC / Diablo Monoclonal Antibody at dilution of 1:10000. Lane A: Hela Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate, Lane C: A431 Whole Cell Lysate, Lane D: MCF7 Whole Cell Lysate, Lysates/proteins at 30 μg per lane.
Western Blot with SMAC / Diablo Monoclonal Antibody at dilution of 1:10000. Lane A: Hela Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate, Lane C: A431 Whole Cell Lysate, Lane D: MCF7 Whole Cell Lysate, Lysates/proteins at 30 μg per lane.
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Overview

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Product Name
Recombinant SMAC/Diablo Monoclonal Antibody
Catalog Number
RD269471A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Rabbit

Background

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This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-61. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen Information

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Immunogen
Recombinant Human SMAC / Diablo protein
Swissprot
Q9NR28
Synonyms
DFNADIABLODFNA64SMAC0610041G12RikDBLOHDBOHDiablo homologDiablo homolog (Drosophila)Diablo homolog mitochondrialDiablo IAP binding mitochondrial proteinDiablo like proteinDIABLO SDirect IAP binding protein with low pIDirect IAP-binding protei
Calculated MW
22 kDa
Observed MW
20 kDa

Applications

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Reactivity
Human
Tested Applications
WB,FC,ICC/IF
Conjugation
Unconjugated
Dilution
WB 1:10000-1:50000;FC 1:25-1:100;ICC/IF 1:20-1:100;
Concentration
1 mg/mL
Storage Buffer
0.2 μm filtered solution in PBS
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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