Recombinant STUB1 Monoclonal Antibody

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Catalog Number
RD269512A
Flow cytometric analysis of Human STUB1 expression on Hela cells. The cells were stained with purified anti-Human STUB1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Flow cytometric analysis of Human STUB1 expression on Hela cells. The cells were stained with purified anti-Human STUB1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence analysis of STUB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human STUB1 Monoclonal Antibody (dilution ratio 1:300) at 4℃ overnight. Then cells were stained with the AF488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Nucleus.
Immunofluorescence analysis of STUB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human STUB1 Monoclonal Antibody (dilution ratio 1:300) at 4℃ overnight. Then cells were stained with the AF488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Nucleus.
Immunoprecipitation analysis using 2 µL anti-STUB1 Monoclonal Antibody and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using STUB1 Monoclonal Antibody at a dilution of 1:500. Lane A:0.5 mg Hela Whole Cell Lysate, Lane B:0.5 mg 293T Whole Cell Lysate, Lane C:0.5 mg MCF-7 Whole Cell Lysate
Immunoprecipitation analysis using 2 µL anti-STUB1 Monoclonal Antibody and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using STUB1 Monoclonal Antibody at a dilution of 1:500. Lane A:0.5 mg Hela Whole Cell Lysate, Lane B:0.5 mg 293T Whole Cell Lysate, Lane C:0.5 mg MCF-7 Whole Cell Lysate
Western Blot with STUB1 Monoclonal Antibody at dilution of 1:500. Lane A: HELA Whole Cell Lysate, Lane B: 293T Whole Cell Lysate, Lane C: MCF7 Whole Cell Lysate, Lysates/proteins at 30 μg per lane.
Western Blot with STUB1 Monoclonal Antibody at dilution of 1:500. Lane A: HELA Whole Cell Lysate, Lane B: 293T Whole Cell Lysate, Lane C: MCF7 Whole Cell Lysate, Lysates/proteins at 30 μg per lane.
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Overview

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Product Name
Recombinant STUB1 Monoclonal Antibody
Catalog Number
RD269512A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Rabbit

Background

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This gene encodes a protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. The encoded protein binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets. Mutations in this gene cause spinocerebellar ataxia, autosomal recessive 16. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 2.

Immunogen Information

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Immunogen
Recombinant Human STUB1 protein
Swissprot
Q9UNE7
Synonyms
STUBSCARUBOXAntigen NY-COCLL-Associated Antigen KWSTIP1 Homology and U Box-Containing ProteinNY-COHSPABPSDCCAGSTUB1CHIPHSPABP2NY-CO-7SCAR16SDCCAG7UBOX1Antigen NY-CO-7Carboxy Terminus of Hsp70-Interacting ProteinCLL-Associated Antigen KW-
Calculated MW
35 kDa
Observed MW
36 kDa

Applications

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Reactivity
Human
Tested Applications
WB,FC,ICC/IF,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000;FC 1:100-1:500;ICC/IF 1:100-1:500;IP 1-4 μL/mg of lysate
Concentration
1 mg/mL
Storage Buffer
0.2 μm filtered solution in PBS
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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