Recombinant TNFR1/CD120a/TNFRSF1A Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Immunofluorescence analysis of Human TNFRI in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TNFRI monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the AF488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Immunofluorescence analysis of Human TNFRI in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TNFRI monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the AF488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
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Overview

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Product Name
Recombinant TNFR1/CD120a/TNFRSF1A Monoclonal Antibody
Catalog Number
RD269747A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG

Background

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TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.

Immunogen Information

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Immunogen
Recombinant Human TNFR1/CD120a/TNFRSF1A Protein
Swissprot
P19438
Synonyms
TBPTumor necrosis factor receptortumor necrosis factor receptor typetumor necrosis factor binding proteinTNFR1-dTNFARMGCTNFR1TBPTNFRSF1ACD120aFPFTBP1TNF-RTNF-R-ITNF-R55TNFARTNFR1TNFR55TNFR60p55p55-Rp60MS5TNFR1-d2TNFARMGC19588TNF-R1

Applications

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Reactivity
Tested Applications
Conjugation
Unconjugated
Dilution
ICC/IF 1:20-1:100
Concentration
1 mg/mL
Storage Buffer
0.2 μm filtered solution in PBS
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

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