Recombinant TROP2/TACSTD2 Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Immunofluorescence analysis of TROP2 in SKBR3 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human TROP2 monoclonal antibody (dilution ratio 1:60) at 4℃ overnight. Then cells were stained with the AF488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to.
Immunofluorescence analysis of TROP2 in SKBR3 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human TROP2 monoclonal antibody (dilution ratio 1:60) at 4℃ overnight. Then cells were stained with the AF488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to.
Immunofluorescence analysis of TROP-2 in NCI-N87 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human TROP-2 monoclonal antibody (dilution ratio 1:60) at 4℃ overnight. Then cells were stained with the AF488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cell membrane.
Immunofluorescence analysis of TROP-2 in NCI-N87 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human TROP-2 monoclonal antibody (dilution ratio 1:60) at 4℃ overnight. Then cells were stained with the AF488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cell membrane.
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Overview

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Product Name
Recombinant TROP2/TACSTD2 Monoclonal Antibody
Catalog Number
RD269731A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG

Background

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This intronless gene encodes a carcinoma-associated antigen. This antigen is a cell surface receptor that transduces calcium signals. Mutations of this gene have been associated with gelatinous drop-like corneal dystrophy.

Immunogen Information

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Immunogen
Recombinant Human TROP2/TACSTD2 Protein
Swissprot
P09758
Synonyms
M1STROPCell surface glycoprotein TropTumor-associated calcium signal transducerMembrane component chromosome 1 surface markerGA733TACSTDEGPTACSTD2EGP-1EGP1GA733-1GA7331GP50M1S1TROP2Cell surface glycoprotein Trop-2Membrane component chromo

Applications

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Reactivity
Tested Applications
Conjugation
Unconjugated
Dilution
ICC/IF 1:20-1:100
Concentration
1 mg/mL
Storage Buffer
0.2 μm filtered solution in PBS
Storage Instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Research Areas

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