Antibody Applications Guide

Apr 30, 2024

Antibodies can be used for a wide range of applications for specific experiments. Understanding how to select, validate, and properly use antibodies is crucial for obtaining reliable and reproducible results. Reddot Biotech offers a large catalog of different antibodies that can be used in a variety of applications.

Use this guide to understand what applications each antibody can be used for.

Abbreviationspace	Term	Definition
Activ	Activation	Inducing a biological response in cells or organisms using antibodies.
	Apoptosis	Programmed cell death process; antibodies can detect or induce apoptosis.
	Binding	Specific attachment of an antibody to its target antigen.
Block	Blocking	Inhibiting a specific biological interaction or activity using antibodies.
Cell Sep-Neg	Cell Separation by Negative Selection	Removing unwanted cells using antibodies targeting specific markers.
ChIP	Chromatin Immunoprecipitation	A type of IP (immunoprecipitation) to study the interaction between proteins and DNA in cells.
	Depletion	Reducing or removing specific cell populations or molecules with antibodies.
ELISA	Enzyme-linked immunosorbent assay	Biochemical assay to detect proteins using antibodies for capture and detection.
FA	Functional Assay	Experiment to assess the functional activity of proteins using antibodies.
FC	Flow Cytometry	Analyzing and sorting cells based on properties, often using fluorescent antibodies.
ICC	Immunocytochemistry	Visualizing proteins in cells using fluorescently labeled antibodies.
ICC/IF	Immunocytochemistry/Immunofluorescence	ICC/IF involves performing ICC (Immunocytochemistry) while specifically using fluorescent labels for visualization.
ICFC	Intracellular Flow Cytometry	Detecting intracellular proteins in flow cytometry using antibodies after cell permeabilization.
IF	Immunofluorescence	Microscopy technique using fluorescent antibodies to visualize proteins in tissues/cells.
IHC	Immunohistochemistry	Visualizing proteins in tissue samples using antibodies.
IHC-p	Immunohistochemistry-Paraffin	IHC technique for formalin-fixed, paraffin-embedded tissue samples.
	Inhibition	Blocking or suppressing a biological process using antibodies.
IP	Immunoprecipitation	Isolating specific proteins from a mixture using antibodies.
Neut	Neutralization	Experimental method used to detect and quantify virus neutralizing antibodies.
	Ready To Use For IHC-P	Pre-optimized antibodies for immediate use in IHC on paraffin-embedded tissues.
Stim	Stimulation	Inducing or enhancing a biological response using antibodies.
WB	Western Blotting	Detecting and analyzing proteins based on size and charge using antibodies.

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Browse our full antibody catalog here.

FAQ

What is the purpose of using antibodies in ELISA?

In ELISA (Enzyme-linked immunosorbent assay), antibodies are used for capturing and detecting proteins, allowing for the biochemical detection of specific proteins in a sample.

How can antibodies be used in flow cytometry?

In flow cytometry, antibodies are often used in a fluorescent form to analyze and sort cells based on their properties, such as the presence of specific proteins on the cell surface or intracellularly.

What is the difference between Immunocytochemistry (ICC) and Immunohistochemistry (IHC)?

Immunocytochemistry (ICC) involves visualizing proteins in cells using fluorescently labeled antibodies, while Immunohistochemistry (IHC) is used for visualizing proteins in tissue samples, often using antibodies to detect proteins in formalin-fixed, paraffin-embedded tissues.

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