Cell Cycle Kit

Understanding the dynamics of the cell cycle is essential for studying cell growth, proliferation, and apoptosis. Our Cell Cycle Assay Kit provides a reliable and easy-to-use method for analyzing cell cycle distribution using flow cytometry. This kit enables accurate quantification of cells in the G₀/G₁, S, and G₂/M phases, as well as identification of apoptotic cells through the detection of sub-G1 DNA content.

Designed for use with a variety of cultured cells, this assay is ideal for researchers studying cancer biology, drug screening, cell cycle regulation, and apoptosis.

Key Features:

Quantitative analysis of G₀/G₁, S, and G₂/M cell cycle phases
Detection of apoptotic cells via sub-G1 DNA content
Nucleic acid dye binds selectively to DNA for accurate fluorescence signal
Includes RNase treatment to eliminate RNA interference
Compatible with standard flow cytometry equipment
Fast, streamlined protocol suitable for high-throughput analysis

How It Works:

In healthy cells, DNA content varies predictably during the cell cycle:

G₀/G₁ phase: Normal diploid DNA content
S phase: DNA synthesis and increasing DNA content
G₂/M phase: Doubled DNA content prior to cell division

This kit uses a DNA-binding fluorescent dye that allows researchers to measure DNA content in individual cells. After treating the cells with RNase to degrade interfering RNA, the dye binds specifically to DNA, and the fluorescence intensity can be measured using flow cytometry.

In apoptotic cells, DNA fragmentation occurs due to the activation of endogenous nucleases. This degradation results in a measurable sub-G1 peak during flow cytometry analysis, allowing researchers to distinguish apoptotic from healthy cells.

Applications:

Cancer research: Monitor cell proliferation and identify disruptions in cell cycle progression
Apoptosis studies: Quantify sub-G1 DNA content to evaluate programmed cell death
Drug discovery and screening: Evaluate compound effects on cell cycle checkpoints
Cell biology: Study cell cycle regulation in response to environmental or genetic factors
Toxicology: Assess the impact of toxins on cell proliferation and cycle arrest

Check out our Cell Cycle Kit here.