Repeating boxes of Reddot Biotech ELISA kits

 

ELISA Kits

ELISA kits (enzyme-linked immuno-sorbent assay) are a common laboratory test to detect and quantify a target protein or small molecule. ELISA kits make use of interactions between antibodies, the target, and signal molecules for detection. The strength of the detection signal allows the user to quantify the amount of target in their samples. 

Reddot Biotech offers ELISA kits in Sandwich or Competitive inhibition format. 

In the sandwich ELISA format, the microtiter plate has been pre-coated with an antibody specific to the target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the target antigen. Next, Avidin conjugated to Horseradish Peroxidase is added to each microplate well and incubated. When the TMB substrate solution is added to the wells, the colour change that occurs is proportional to the concentration of target antigen in the sample. The standard curve is used to calculate the concentration.

Image showing the binding interactions between antibodies, antigen, and signal molecules in a sandwich elisa kit

In the competitive inhibition ELISA format, the microtiter plate is precoated with monoclonal antibodies, or antigens, that are specific to the target antigen. This monoclonal antibody (or antigen) has ONE binding site. The antigen from your sample competes with an inhibitor antigen conjugated with biotin. If the biotin-conjugated inhibitor antigen complex binds to a monoclonal antibody, this interaction will produce signal. If the antigen from the sample binds to a monoclonal antibody, this interaction will NOT produce signal. Next, Avidin conjugated to Horseradish Peroxidase is added to each microplate well and incubated. The amount of bound horseradish peroxidase conjugate is reverse proportional to the concentration of the target antigen in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the target antigen in the sample. The standard curve is used to calculate the concentration.

Image showing the binding interactions of the competitor antigen in a competitive inhibition ELISA kit

 

Reddot Biotech also offers all ELISA kits in Traditional or Ready-To-Use Format. Our updated Ready-To-Use line has pre-diluted detection reagents, shortened incubation times, and enhanced stability for a 16 month shelf life. Both ELISA kits lines maintain our high level of quality and sensitivity. 

Table showing the differences between ELISA kits from Reddot Biotech

Browse our Traditional ELISA kits here.

Browse our Ready-To-Use ELISA kits here.

 

Reddot Biotech also offers customization options by request for different targets or sample type optimization. Contact us with your request for more information. 

 

 

Antibodies

We also offer a large range of Monoclonal, Polyclonal, Secondary and Flow Cytometry antibodies to complement our ELISA kit catalogue.

Browse all Antibodies here.

 

Featured ELISA Kits

Our current featured ELISA kits are the five members of the human VEGF (vascular endothelial growth factor) protein family. VEGF (also known as VEGF-A or VEGFA) is a signalling protein involved in the formation of blood vessels. Check out our blog post on these kits with more in-depth information about the target analytes and their functions.

Catalogue Number: RDR-VEGFA-Hu 
Detection Range: 15.6-1000pg/mL
Sensitivity: 6.1pg/mL
Synonyms: VEGF, VEGF-A, VPF, Vascular Endothelial Cell Growth Factor A, Vascular Permeability Factor
 
Catalogue Number: RDR-VEGFB-Hu 
Detection Range: 15.6-1000pg/mL
Sensitivity: 6.3pg/mL
Synonyms: VEGF-B, VEGFL, VRF, VEGF-related factor
 
Catalogue Number: RDR-VEGFC-Hu 
Detection Range: 62.5-4000pg/mL
Sensitivity: 23.7pg/mL
Synonyms: VEGFC, Flt4-L, VRPL, Vascular Endothelial Growth Factor-Related Protein
 
Catalogue Number: RDR-VEGFD-Hu 
Detection Range: 15.6-1000pg/mL
Sensitivity: 6.1pg/mL
Synonyms: VEGF-D, FIGF, C-Fos Induced Growth Factor
 
Catalogue Number: RDR-PLGF-Hu 
Detection Range: 15.6-1000pg/mL
Sensitivity: 5.7pg/mL
Synonyms: PlGF2, PGF, PGFL, Placental Growth Factor-Like, Vascular Endothelial Growth Factor-Related Protein

 

Browse all VEGF related ELISA kits here.

 

All components of a Ready-To-Use ELISA kit from Reddot Biotech

Browse all ELISA kits here.

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