Western blot analysis of extracts of various cell lines using ACACA Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of 293T cells using ACACA Polyclonal Antibody at dilution of 1:100. 293T cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight(left). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using ACACA Polyclonal Antibody at dilution of 1:100. C6 cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight(left). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using ACACA Polyclonal Antibody at dilution of 1:100. HeLa cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight(left). Blue: DAPI for nuclear staining.
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.