ELISA (enzyme-linked immunosorbent assay) is a common laboratory technique used to detect and quantify the amount of analyte in a given sample. 
EASYStep Pro ELISA kits are an optimized version of our EASYStep ELISA kits that feature an even shorter experimental time and only two incubation steps.

Browse EASYStep Pro ELISA Kits

  • Even shorter experimental time - done in 1.5 hours!
  • Very easy procedure - only 2 incubation steps
  • Small sample volume - only 50uL needed
  • Available for 62 popular targets
  • Easy storage - store at 4oC for up to 6 months
EASYStep Pro ELISA Kit Components:
  • Pre-coated 96-well micro ELISA plate 
  • 2 Standards
  • Diluent Buffer Pro
  • Wash Buffer (25× concentrate) 
  • Detection Reagent Pro (100x concentrate)
  • Detection Reagent Diluent
  • Substrate Reagent
  • Stop Solution 
  • 5 Plate sealers 
  • Instruction manual
EASYStep Pro ELISA Kit Procedure:
  1. Add 50 µL standard or sample to each well, immediately add add 50 μL of Detection Reagent Pro working solution to each well. 
    Incubate for 60 minutes at 37°C.
  2. Aspirate and wash the plate for 5 times. 
  3. Add 90 µL Substrate Solution. Incubate for 15 minutes at 37°C. 
  4. Add 50 µL Stop Solution. Read at 450 nm immediately.
Choose your fastest ELISA yet with EASYStep Pro ELISA Kits

Reddot Biotech ELISA Formats

  Traditional Ready-To-Use EASYStep EASYStep Pro
Experimental time (Assay Length) 4.5 Hours 3.5 Hours 2.5 Hours 1.5 Hours
Storage Separate -20 °C and 4 °C -20 °C 4 °C 4 °C
Pre-Diluted Detection Reagents? No Yes No No
Target Catalogue 6000+ 6000+ 20 62
Sample Volume 100uL 100uL 50uL 50uL
Incubation Steps 4 4 3 2

Why Reddot ELISAs?

When you choose Reddot Biotech products, you’re choosing high quality, research-ready reagents specifically designed to help your experiments succeed. We believe in supporting scientists on their way to the next big breakthrough to make the world a better place. If you have any questions or concerns, or just want some advice, we are always here to help.

ELISAs Resources

Performance Guarantee

All Reddot Biotech ELISA kits come with a performance quality guarantee. Contact us if you are unsatisfied with your product.

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