EASYStep Pro ELISA Kits

ELISA (enzyme-linked immunosorbent assay) is a common laboratory technique used to detect and quantify the amount of analyte in a given sample. 
EASYStep Pro ELISA kits are an optimized version of an instant ELISA that feature a very short experimental time and only two incubation steps for maximum convenience and efficiency.

Browse EASYStep Pro ELISA Kits


EASYStep Pro ELISA Kits are revolutionary instant immunoassay solutions designed for researchers who need fast, reliable, and easy-to-use ELISA assays. Unlike traditional ELISA kits that require multiple incubation and washing steps, EASYStep Pro ELISA Kits streamline the process with only two incubation steps, significantly reducing hands-on time. With a total experimental time of just 1.5 hours, these kits provide quick and reproducible results for a variety of research and diagnostic applications.

EASYStep Pro ELISA Kits are available for 62 popular targets, covering cytokines, growth factors, biomarkers, and more. Designed for convenience, these kits require only 50µL of sample volume and can be stored at 4°C for up to 6 months, eliminating the need for ultra-low temperature storage.


Key Features & Benefits

  • Ultra-Fast Experimental Time – Complete the entire experiment in just 1.5 hours.
  • Simplified Protocol – Only two incubation steps, heavily reducing handling time.
  • Minimal Sample Required – Requires just 50µL per well, conserving precious samples.
  • Broad Target Selection – Available for 62 high-demand biomarkers.
  • Easy Storage & Long Shelf Life – Stable at 4°C for up to 6 months.

Applications for EASYStep Pro ELISA Kits

EASYStep Pro ELISA Kits are ideal for:

  • Biomedical Research – Quick quantification of cytokines, hormones, and proteins.
  • Clinical & Disease Studies – Fast biomarker detection for disease research.
  • Pharmaceutical & Drug Development – High-throughput screening and pharmacokinetic studies.
  • Veterinary & Agricultural Research – Efficient analysis of biological samples.

How EASYStep Pro ELISAs Work – Simple 3-Step Workflow

  1. Add Sample & Incubate – Pipette 50µL of your sample into the pre-coated microplate and incubate.
  2. Add Detection Reagent & Incubate – Introduce the detection solution and incubate again.
  3. Wash, Add Substrate & Read Results – Perform simple washes, add substrate, and measure absorbance using a microplate reader.
  4. That's it! 

With this streamlined approach, EASYStep Pro ELISA Kits eliminate unnecessary steps while maintaining high sensitivity and specificity.


EASYStep Pro ELISA Kit Components:

  • Pre-coated 96-well micro ELISA plate 
  • 2 Standards
  • Diluent Buffer Pro
  • Wash Buffer (25× concentrate) 
  • Detection Reagent Pro (100x concentrate)
  • Detection Reagent Diluent
  • Substrate Reagent
  • Stop Solution 
  • 5 Plate sealers 
  • Instruction manual

Frequently Asked Questions (FAQs)

Q: How do EASYStep Pro ELISA kits compare to traditional ELISA kits?
A: EASYStep Pro ELISA Kits require only two incubation steps and take just 1.5 hours to complete, compared to traditional ELISA kits that may take 3–5 hours and involve multiple wash steps.

Q: What sample types can be used with EASYStep Pro ELISA Kits?
A: These kits are compatible with serum, plasma, cell culture supernatants, and other biological fluids depending on the target analyte.

Q: How should I store EASYStep Pro ELISA Kits?
A: Store the kits at 4°C for up to 6 months, making them convenient and easy to handle without requiring freezing.

Q: Are EASYStep Pro ELISA Kits as sensitive as traditional ELISA kits?
A: Yes! Despite the faster protocol, these kits maintain high sensitivity and specificity, ensuring reliable and reproducible results.


Explore Our EASYStep Pro ELISA Kits

Discover the fastest and most convenient way to perform ELISA assays with EASYStep Pro ELISA Kits. Browse our selection of 62 high-demand targets and experience high-performance immunoassays in just 1.5 hours. Have questions? Contact our expert support team for assistance!

Choose your fastest ELISA experience yet with EASYStep Pro ELISA Kits!


Featured ELISA Kits VIEW ALL


Reddot Biotech ELISA Formats

  Traditional Ready-To-Use EASYStep Pro
Experimental time (Assay Length) 4.5 Hours 3.5 Hours 1.5 Hours
Storage Separate -20 °C and 4 °C -20 °C 4 °C
Pre-Diluted Detection Reagents? No Yes No
Target Catalogue 6000+ 6000+ 62
Sample Volume 100uL 100uL 50uL
Incubation Steps 4 4 2

Why Reddot ELISAs?

When you choose Reddot Biotech products, you’re choosing high quality, research-ready reagents specifically designed to help your experiments succeed. We believe in supporting scientists on their way to the next big breakthrough to make the world a better place. If you have any questions or concerns, or just want some advice, we are always here to help.


ELISAs Resources

Performance Guarantee

All Reddot Biotech ELISA kits come with a performance quality guarantee. Contact us if you are unsatisfied with your product.

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