Ready-To-Use ELISA Kits

Ready-to-Use ELISA (Enzyme-Linked Immunosorbent Assay) kits are pre-assembled immunoassay solutions designed to simplify laboratory workflows. ELISA is a widely used biochemical technique that detects and quantifies specific analytes (often proteins, peptides, or small molecules) in biological samples. 

Ready-to-Use ELISA kits come with pre-coated plates, optimized reagents, and easy-to-follow protocols, reducing preparation time and minimizing variability. These features make them ideal for researchers and laboratory professionals who require fast, accurate, and reproducible results.

Browse Ready-To-Use ELISA Kit

• Expansive Target Catalog – over 6500 different kits
• Pre-Coated & Pre-Assembled – No need for manual coating or reagent optimization.
• Optimized Reagents – Ensures high sensitivity and specificity.
• Easy-to-Follow Protocols – Reduces user errors and improves consistency.
• Pre-diluted Detection Reagents – one less prep step
• Faster Turnaround – Shorter incubation times and fewer experimental steps.
• Compatible with Multiple Sample Types – Works with serum, plasma, cell lysate, and much more!
• Enhanced Stability – store at -20^oC for up to 16 months

Applications for Ready-to-Use ELISA Kits

These kits can be widely used in various research and industrial applications, including:

• Biomedical Research – Quantification of cytokines, hormones, and biomarkers.
• Clinical Research – Detection of disease markers and infectious agents.
• Pharmaceutical Research – Drug discovery, pharmacokinetics, and therapeutic monitoring.

How to Choose the Right Kit

When selecting the best kit for your needs, consider the following factors:

• Target Analyte – Find the correct target for your experiment, whether that's a protein, peptide, or small molecule.
• Sample Compatibility – Ensure the kit supports your desired sample type (e.g., serum, plasma, cell culture supernatant).
• Detection Method – All Reddot Biotech kits use colorimetric detection for simplicity.
• Assay Format – Make sure the listed format (sandwich or competitive inhibition) fits your needs, or contact us to develop a custom solution!

• Pre-coated 96-well strip plate
• 2 Plate sealers for 96 wells 
• 2 Standards
• Standard Diluent 
• Wash Buffer (30× concentrate)
• Detection Solution A  
• Detection Solution B 
• TMB Substrate 
• Stop Solution 
• Instruction manual

Ready-To-Use ELISA Kit Procedure:

1. Prepare all reagents, samples and standards;
2. Add 100 µL standard or sample to each well. Incubate 90 minutes at 37°C;
3. Aspirate and add 100 µL Detection Solution A. Incubate 45 minutes at 37°C;
4. Aspirate and wash 3 times;
5. Add 100 µL Detection Solution B. Incubate 45 minutes at 37°C; 
6. Aspirate and wash 5 times;
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37°C;
8. Add 50 µL Stop Solution. Read at 450 nm immediately.

Frequently Asked Questions (FAQs)

Q: What makes Ready-to-Use ELISA kits different from traditional ELISA kits?
A: Ready-to-Use ELISA kits come with pre-diluted detection reagents, shorter incubation times, and a longer shelf life, reducing preparation time and minimizing variability, and making them more efficient and reliable.

Q: How should I store and handle Ready-To-Use ELISA kits?
A: Always store kits as directed in the manual or on labels. For Ready-To-Use ELISA kits from Reddot Biotech, you can store all components at -20^oC for up to 16 months.

Q: What sample types can be used with Ready-to-Use ELISA kits?
A: These kits are compatible with serum, plasma, cell lysate, cell culture supernatant, and other biological fluids, depending on the assay. Check the manual for further details or contact us for a customized solution to your needs. 

Explore Our ELISA Kit Selection

Browse our comprehensive range of Ready-To-Use ELISA Kits to find the best option for your research needs. Need assistance? Contact our support team for expert guidance, or explore our other ELISA kit ranges here:

Traditional ELISA Kits

EASYStep Pro ELISA Kits

Food Safety ELISA Kits