Ready-To-Use ELISA Kits

ELISA (enzyme-linked immunosorbent assay) is a common laboratory technique used to detect and quantify the amount of analyte in a given sample. 
The Ready-To-Use ELISA kit is an optimized version of our Traditional kit that offers pre-diluted detection reagents, a shorter experimental time, and enhanced stability for a 16-month shelf life. 

Browse Ready-To-Use ELISA Kit

  • Expansive target catalogue - over 6500 different kits
  • Straightforward procedure - easy-to-follow steps
  • Pre-diluted detection reagents - one less prep step
  • Shorter experimental time - done in 3.5 hours
  • Enhanced stability - store at -20oC for up to 16 months
Ready-To-Use ELISA Kit Components:
  • Pre-coated 96-well strip plate
  • 2 Plate sealers for 96 wells 
  • 2 Standards
  • Standard Diluent 
  • Wash Buffer (30× concentrate)
  • Detection Solution A  
  • Detection Solution B 
  • TMB Substrate 
  • Stop Solution 
  • Instruction manual

Ready-To-Use ELISA Kit Procedure:

  1. Prepare all reagents, samples and standards;
  2. Add 100 µL standard or sample to each well. Incubate 90 minutes at 37°C;
  3. Aspirate and add 100 µL Detection Solution A. Incubate 45 minutes at 37°C;
  4. Aspirate and wash 3 times;
  5. Add 100 µL Detection Solution B. Incubate 45 minutes at 37°C; 
  6. Aspirate and wash 5 times;
  7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37°C;
  8. Add 50 µL Stop Solution. Read at 450 nm immediately.

Reddot Biotech ELISA Formats

  Traditional Ready-To-Use EASYStep EASYStep Pro
Experimental time (Assay Length) 4.5 Hours 3.5 Hours 2.5 Hours 1.5 Hours
Storage Separate -20 °C and 4 °C -20 °C 4 °C 4 °C
Pre-Diluted Detection Reagents? No Yes No No
Target Catalogue 6000+ 6000+ 20 62
Sample Volume 100uL 100uL 50uL 50uL
Incubation Steps 4 4 3 2

Why Reddot ELISAs?

When you choose Reddot Biotech products, you’re choosing high quality, research-ready reagents specifically designed to help your experiments succeed. We believe in supporting scientists on their way to the next big breakthrough to make the world a better place. If you have any questions or concerns, or just want some advice, we are always here to help.

ELISAs Resources

Performance Guarantee

All Reddot Biotech ELISA kits come with a performance quality guarantee. Contact us if you are unsatisfied with your product.

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