ELISA (enzyme-linked immunosorbent assay) is a common laboratory technique used to detect and quantify the amount of analyte in a given sample. 
EASYStep ELISA kits are a form of 'instant ELISAs' with a shorter workflow and much less hands-on and incubation time required compared to conventional ELISA kits. 

Browse EASYStep ELISA Kits

  • Shorter experimental time - done in 2.5 hours
  • Easy procedure - only 3 incubation steps
  • Small sample volume - only 50uL needed
  • Available for 20 popular targets
  • Easy storage - store at 4oC for up to 6 months
EASYStep ELISA Kit Components:
  • Pre-coated 96-well micro ELISA plate
  • 5 Plate sealers 
  • 2 Standards
  • Diluent Buffer
  • Wash Buffer (25× concentrate) 
  • Detection Solution  
  • HRP Conjugate (100x concentrate) 
  • HRP Conjugate Diluent
  • Substrate Reagent
  • Stop Solution 
  • Instruction manual
EASYStep ELISA Kit Procedure:
  1. Add 50 µL standard or sample to each well, immediately add 50 µL Detection Solution to each well. Incubate for 90 minutes at 37°C.
  2. Aspirate and wash the plate for 3 times. 
  3. Add 100 µL HRP conjugate working solution. Incubate for 30 minutes at 37°C. 
  4. Aspirate and wash 5 times. 
  5. Add 90 µL Substrate Solution. Incubate for 15 minutes at 37°C. 
  6. Add 50 µL Stop Solution. Read at 450 nm immediately.

Reddot Biotech ELISA Formats

  Traditional Ready-To-Use EASYStep EASYStep Pro
Experimental time (Assay Length) 4.5 Hours 3.5 Hours 2.5 Hours 1.5 Hours
Storage Separate -20 °C and 4 °C -20 °C 4 °C 4 °C
Pre-Diluted Detection Reagents? No Yes No No
Target Catalogue 6000+ 6000+ 20 62
Sample Volume 100uL 100uL 50uL 50uL
Incubation Steps 4 4 3 2

Why Reddot ELISAs?

When you choose Reddot Biotech products, you’re choosing high quality, research-ready reagents specifically designed to help your experiments succeed. We believe in supporting scientists on their way to the next big breakthrough to make the world a better place. If you have any questions or concerns, or just want some advice, we are always here to help.

ELISAs Resources

Performance Guarantee

All Reddot Biotech ELISA kits come with a performance quality guarantee. Contact us if you are unsatisfied with your product.

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