Traditional ELISA Kits

ELISA (enzyme-linked immunosorbent assay) is a common laboratory technique used to detect and quantify the amount of analyte in a given sample. The Traditional ELISA kit is our foundational ELISA kit that has a conventional workflow at a reasonable price, while maintaining the same high level of quality and sensitivity.

Browse Traditional ELISA Kits

  • Expansive target catalogue - over 6500 different kits
  • Many different reactivities available -including hard to find species
  • Straightforward procedure - easy-to-follow steps
  • Affordable - prices to fit all budgets including large projects
  • Complete - includes everything you need except your samples
Traditional ELISA Kit Components:
  • Pre-coated 96-well strip plate
  • 2 Plate sealers for 96 wells 
  • 2 Standards
  • Diluent Buffer
  • Wash Buffer (30× concentrate)
  • Detection Reagent A  
  • Detection Reagent B 
  • TMB Substrate 
  • Stop Solution 
  • Instruction manual

Traditional ELISA Kit Procedure:

  1. Prepare all reagents, samples and standards;
  2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37°C;
  3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
  4. Aspirate and wash 3 times;
  5. Add 100 µL prepared Detection Reagent B. Incubate 1 hour at 37°C; 
  6. Aspirate and wash 5 times;
  7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37°C;
  8. Add 50 µL Stop Solution. Read at 450 nm immediately.

Reddot Biotech ELISA Formats

  Traditional Ready-To-Use EASYStep EASYStep Pro
Experimental time (Assay Length) 4.5 Hours 3.5 Hours 2.5 Hours 1.5 Hours
Storage Separate -20 °C and 4 °C -20 °C 4 °C 4 °C
Pre-Diluted Detection Reagents? No Yes No No
Target Catalogue 6000+ 6000+ 20 62
Sample Volume 100uL 100uL 50uL 50uL
Incubation Steps 4 4 3 2

Why Reddot ELISAs?

When you choose Reddot Biotech products, you’re choosing high quality, research-ready reagents specifically designed to help your experiments succeed. We believe in supporting scientists on their way to the next big breakthrough to make the world a better place. If you have any questions or concerns, or just want some advice, we are always here to help.

ELISAs Resources

Performance Guarantee

All Reddot Biotech ELISA kits come with a performance quality guarantee. Contact us if you are unsatisfied with your product.

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