Active CASP3 Monoclonal Antibody
Catalog Number
RD85535A
Western Blot analysis of 1) Hela, 2) 3T3, 3) Rat brain with Active CASP3 Monoclonal Antibody
Immunohistochemistry of paraffin-embedded Human liver tissue using Active CASP3 Monoclonal Antibody at dilution of 1:200.
Variation
Quantity
For more information on our kits, please contact us at info@reddotbiotech.com
Overview
Product Name
Active CASP3 Monoclonal Antibody
Catalog Number
RD85535A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
Immunogen Information
Immunogen
Recombinant Protein
Swissprot
P42574
Synonyms
A830040C14RikApopainCASP-3CASP3CASP3Casp3aCaspase 3Caspase 3apoptosis-related cysteine peptidaseCaspase 3apoptosis-related cysteine proteaseCaspase 3apoptosis-related cysteine protease aCaspase-3 subunit p12CC3CPP-32CPP32CPP32BCysteine p
Observed MW
17 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:100-1:200, IF 1:100-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas