alpha Tubulin Polyclonal Antibody
Catalog Number
RD86534A
Western Blot analysis of Hela, A549 cells using alpha Tubulin Polyclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using alpha Tubulin Polyclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Human lung tissue using alpha Tubulin Polyclonal Antibody at dilution of 1:200.
Variation
Quantity
For more information on our kits, please contact us at info@reddotbiotech.com
Overview
Product Name
alpha Tubulin Polyclonal Antibody
Catalog Number
RD86534A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.
Immunogen Information
Immunogen
Synthesized peptide derived from the C-terminal region of human Tubulin α
Swissprot
Q71U36,P68363
Synonyms
Alpha tubulin 3Alpha-tubulin 3B alpha 1FLJ25113LIS3TBA1ATUBA1ATUBA3Tubulin alpha 1aTubulin alpha 1A chainTubulin alpha 3Tubulin alpha 3 chainTubulin alpha brain specificTubulin alpha-1A chainTubulin alpha-3 chainTubulin B alpha 1Tubulin B-
Calculated MW
50 kDa
Observed MW
50 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas