AMPK alpha1 Monoclonal Antibody
Catalog Number
RD85546A
Western Blot analysis of 1) Hela, 2) 293T, 3) 3T3, 4) PC-12 cells using AMPK alpha1 Monoclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Mouse colon tissue using AMPK alpha1 Monoclonal Antibody at dilution of 1:200.
Variation
Quantity
For more information on our kits, please contact us at info@reddotbiotech.com
Overview
Product Name
AMPK alpha1 Monoclonal Antibody
Catalog Number
RD85546A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Immunogen Information
Immunogen
Synthetic Peptide
Swissprot
Q13131
Synonyms
5 AMP activated protein kinase alpha 1catalytic subunit5 AMP activated protein kinase catalytic alpha 1 chain5' AMP activated protein kinase catalytic subunit alpha 15'-AMP-activated protein kinase catalytic subunit alpha-1AAPK1AAPK1ACACA kinaseace
Observed MW
63 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p
Conjugation
Unconjugated
Dilution
WB 1:1000-2000, IHC 1:50-100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.