BCL-x Polyclonal Antibody
Catalog Number
RD70825A
Western Blot analysis of 293T cells using Bcl-x Polyclonal Antibody at dilution of 1:500.
Variation
Quantity
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Overview
Product Name
BCL-x Polyclonal Antibody
Catalog Number
RD70825A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Bcl x is a member of the Bcl 2 family of proteins, which function as regulators of apoptosis. It may provide neuroprotection against ischemic brian injury. Bcl x has two isoforms, Bcl xl (long) a 241 amino acid protein and Bcl xs (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the Bcl2 family. Isoform Bcl-X(L) anti-apoptotic activity is inhibited by association with SIVA isoform 1. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, cytochrome c, from the mitochondrial membrane. The Bcl-X(S) isoform promotes apoptosis.
Immunogen Information
Immunogen
Synthesized peptide derived from human Bcl-x around the non-phosphorylation site of Thr47.
Swissprot
Q07817
Synonyms
Apoptosis regulator Bcl XApoptosis regulator Bcl-XApoptosis regulator BclXB cell lymphoma 2 likeB2CL1Bcl 2 like 1 proteinBcl XBcl xLBCL XL/SBcl xSBcl-2-like protein 1Bcl2 Like 1Bcl2 related geneBcl2-L-1BCL2LBcl2l1BCLXBclXLBclXsDKFZp781P
Calculated MW
26 kDa
Observed MW
30 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas