beta actin Polyclonal Antibody
Catalog Number
RD86599A
Variation
Quantity
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Overview
Product Name
beta actin Polyclonal Antibody
Catalog Number
RD86599A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.
Immunogen Information
Immunogen
Synthesized peptide derived from the N-terminal region of human Actin β.
Swissprot
P60709
Synonyms
A26C1AA26C1BACTBACTBActin betaActin cytoplasmic 1Actincytoplasmic 1N-terminally processedActxb actinBeta cytoskeletal actinBeta-actinBRWS1E430023M04RikMGC128179PS1TP5 binding protein 1PS1TP5BP1
Calculated MW
42 kDa
Observed MW
42 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,ELISA
Conjugation
Unconjugated
Dilution
WB 1:1000-1:4000, IHC 1:100-1:300, ELISA 1:20000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas