EYA3 Polyclonal Antibody
Catalog Number
RD266408A
Western blot analysis of extracts of various cell lines using EYA3 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of U2OS cells using EYA3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
EYA3 Polyclonal Antibody
Catalog Number
RD266408A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human EYA3 (NP_001981.2).
Gene ID
2140
Swissprot
Q99504
Synonyms
EYA3
Calculated MW
48 kDa/56 kDa/57 kDa/58 kDa/62 kDa
Observed MW
57 kDa/58 kDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.