GADD45A Polyclonal Antibody
Catalog Number
RD265839A
Western blot analysis of extracts of various cell lines using GADD45A Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat lung using GADD45A Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human thyroid cancer using GADD45A Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse liver using GADD45A Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using GADD45A Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Variation
Quantity
For more information on our kits, please contact us at info@reddotbiotech.com
Overview
Product Name
GADD45A Polyclonal Antibody
Catalog Number
RD265839A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human GADD45A (NP_001915.1).
Gene ID
1647
Swissprot
P24522
Synonyms
DDIT1GADD45GADD45A
Calculated MW
14 kDa/18 kDa
Observed MW
20 kDa/25 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas