GLS Polyclonal Antibody
Catalog Number
RD219340A
Western blot analysis of 293T cell lysate using GLS Polyclonal Antibody at dilution of 1:850
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using GLS Polyclonal Antibody at dilution of 1:70(×200)
Immunohistochemistry of paraffin-embedded Human tonsil tissue using GLS Polyclonal Antibody at dilution of 1:70(×200)
Variation
Quantity
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Overview
Product Name
GLS Polyclonal Antibody
Catalog Number
RD219340A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
GLS,also named as GLS1 and KIAA0838,belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-,glutamate-,and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells,in accordance with measurements of glutaminase activity,and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase. It has 3 isoforms named as KGA,GAM,GAC.
Immunogen Information
Immunogen
Synthetic peptide of human GLS
Swissprot
O94925
Synonyms
AAD20DKFZp686O15119FLJ10358GACGAMGLSGLS1GLSKGlutaminase CGlutaminase kidney isoformGlutaminase phosphate activatedK-glutaminaseKGAKIAA0838L-glutamine amidohydrolasemitochondrial
Calculated MW
73 kDa
Observed MW
Refer to figures
Gene Accession
NP001243239
Applications
Reactivity
Human, Mouse, Rat
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:50-1:200, ELISA 1:5000-1:10000
Concentration
1.02 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas