Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
Immunogen Information
Immunogen
Synthesized peptide derived from the Internal region of human HIF-1α.
Swissprot
Q16665
Synonyms
ARNT interacting proteinARNT-interacting proteinBasic helix loop helix PAS protein MOP1Basic-helix-loop-helix-PAS protein MOP1bHLHe78Class E basic helix-loop-helix protein 78HIF 1AHIF 1alphaHIF-1-alphaHIF1 AHIF1 AlphaHIF1HIF1-alphaHIF1AHIF1A
Calculated MW
93 kDa
Observed MW
92-130 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:40000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4