Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
Immunogen Information
Immunogen
6X His synthetic peptide conjugated to KLH.
Synonyms
6 His epitope tagHexa His tagHHHHHH epitope tagHHHHHH tagHis tagPolyhistidine Tag
Observed MW
15 kDa
Applications
Tested Applications
WB,ELISA
Conjugation
Unconjugated
Dilution
WB 1:1000-1:3000, ELISA 1:20000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4