Histone H3 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD71286A
Western Blot analysis of MCF7 cells using Histone H3 Polyclonal Antibody at dilution of 1:2000.
Variation
Quantity
For more information on our kits, please contact us at info@reddotbiotech.com
Overview
Product Name
Histone H3 Polyclonal Antibody
Catalog Number
RD71286A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Immunogen Information
Immunogen
Synthesized peptide derived from human Histone H3 around the non-phosphorylation site of Ser10.
Swissprot
P68431,Q71DI3,P84243
Synonyms
HIST1H3AH3FAHIST1H3BH3FLHIST1H3CH3FCHIST1H3DH3FBHIST1H3EH3FDHIST1H3FH3FIHIST1H3GH3FHHIST1H3HH3FKHIST1H3IH3FFHIST1H3JH3FJHistone H3.1Histone H3/aHistone H3/bHistone H3/cHistone H3/dHistone H3
Calculated MW
15 kDa
Observed MW
20 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:20000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas