LC3B Polyclonal Antibody
Catalog Number
RD80562A
Western blot analysis of extracts of various cell lines using LC3B Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat brain using LC3B Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse brain using LC3B Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of LC3B knockout 293T cells treated by chloroquine (top left) and wild-type 293T cells (top middle)，wild-type 293T cells treated by chloroquine (top right) using LC3B Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Variation
Quantity
For more information on our kits, please contact us at info@reddotbiotech.com
Overview
Product Name
LC3B Polyclonal Antibody
Catalog Number
RD80562A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Immunogen Information
Immunogen
A synthetic peptide of human LC3B (NP_073729.1).
Gene ID
81631
Swissprot
Q9GZQ8
Synonyms
LC3BATG8FMAP1LC3B-aMAP1A/1BLC3MAP1LC3BLC3A/LC3B
Calculated MW
14 kDa
Observed MW
14 kDa/15 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.