Luciferase Monoclonal Antibody
Catalog Number
RD85655A
Western Blot analysis of Firefly Luciferase Transfected Hela using Luciferase Monoclonal Antibody at dilution of 1:2000.
Variation
Quantity
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Overview
Product Name
Luciferase Monoclonal Antibody
Catalog Number
RD85655A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Immunogen Information
Immunogen
Synthetic Peptide of Luciferase
Swissprot
P08659
Synonyms
ec 1.13.12.7FireflyLuciferaseLuciferin 4 monooxygenase
Observed MW
60 kDa
Applications
Reactivity
Firefly
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas