Myc-Tag Monoclonal Antibody

Catalog Number
RD86491A
Western Blot analysis of 1ug Myc fusion protein using Myc-Tag Monoclonal Antibody at dilution of 1) 1:5000 2) 1:10000.
Western Blot analysis of 1ug Myc fusion protein using Myc-Tag Monoclonal Antibody at dilution of 1) 1:5000 2) 1:10000.
Immunofluorescence analysis of 293 cells transfected with a Myc tag protein tissue using Myc-Tag Monoclonal Antibody at dilution of 1:2000.
Immunofluorescence analysis of 293 cells transfected with a Myc tag protein tissue using Myc-Tag Monoclonal Antibody at dilution of 1:2000.
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Overview
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Product Name
Myc-Tag Monoclonal Antibody
Catalog Number
RD86491A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
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Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
Immunogen Information
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Immunogen
Synthetic Peptide
Synonyms
c Mycc Myc Epitope Tagc Myc TagMyc Epitope TagMyc proto-oncogene proteinTranscription factor p64
Applications
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Tested Applications
WB,IF,IP
Conjugation
Unconjugated
Dilution
WB 1:5000-1:10000, IF 1:500-1:2000, IP 1:100-1:300
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas
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