PCNA Monoclonal Antibody
Catalog Number
RD85459A
Western Blot analysis of Hela, Rat brain, NIH, 3T3, 293T using Proliferating Cell Nuclear Antigen Monoclonal Antibody at dilution of 1:5000.
Immunohistochemistry of paraffin-embedded Rat testis tissue using Proliferating Cell Nuclear Antigen Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Rat testis tissue using Proliferating Cell Nuclear Antigen Monoclonal Antibody at dilution of 1:200.
Variation
Quantity
For more information on our kits, please contact us at info@reddotbiotech.com
Overview
Product Name
PCNA Monoclonal Antibody
Catalog Number
RD85459A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
Immunogen Information
Immunogen
Synthetic Peptide
Swissprot
P12004
Synonyms
ATLD2cb16CyclinDNA polymerase delta auxiliary proteinetID36690.10fa28e03fb36g03HGCN8729MGC8367Mutagen-sensitive 209 proteinOTTHUMP00000030189OTTHUMP00000030190PCNAPcna/cyclinPCNAPCNARPolymerase delta accessory proteinProliferating cell nu
Calculated MW
29 kDa
Observed MW
29 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF
Conjugation
Unconjugated
Dilution
WB 1:5000-1:10000, IHC 1:100-1:300, IF 1:100-1:300
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.